rabbit peptide antibody Search Results


antihuman c peptide rabbit igg  (OriGene)
90
OriGene antihuman c peptide rabbit igg
Antihuman C Peptide Rabbit Igg, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/C-Peptide+Rabbit+Polyclonal+Antibody/pmc06496474-77-0-4
Average 90 stars, based on 1 article reviews
antihuman c peptide rabbit igg - by Bioz Stars, 2026-10
90/100 stars
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pyy  (OriGene)
90
OriGene pyy
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Pyy, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Peptide+YY+%2F+PYY+Rabbit+Polyclonal+Antibody/pmc03880604-176-27-29
Average 90 stars, based on 1 article reviews
pyy - by Bioz Stars, 2026-10
90/100 stars
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gcb origene ta351444 wb  (OriGene)
91
OriGene gcb origene ta351444 wb
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Gcb Origene Ta351444 Wb, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Natriuretic+Peptide+Receptor+B+(NPR2)+Rabbit+Polyclonal+Antibody/pmc10396312__sciadv__add4222_sm-131-22-23
Average 91 stars, based on 1 article reviews
gcb origene ta351444 wb - by Bioz Stars, 2026-10
91/100 stars
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rabbit igg  (Valiant Co Ltd)
96
Valiant Co Ltd rabbit igg
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Rabbit Igg, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Anti-glucagon+rabbit+polyclonal+antibody%2C+250+%C2%B5L/pm41730615-56-35-37
Average 96 stars, based on 1 article reviews
rabbit igg - by Bioz Stars, 2026-10
96/100 stars
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rabbit antihuman c peptide  (RayBiotech inc)
90
RayBiotech inc rabbit antihuman c peptide
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Rabbit Antihuman C Peptide, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Rabbit+Anti-Human+C-Peptide/10__14233_slash_ajchem__2014__17541-46-24-33
Average 90 stars, based on 1 article reviews
rabbit antihuman c peptide - by Bioz Stars, 2026-10
90/100 stars
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ap02242su n  (OriGene)
91
OriGene ap02242su n
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Ap02242su N, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Tuberoinfundibular+peptide+(PTH2)+Rabbit+Polyclonal+Antibody/pmc10450825-89-9-7
Average 91 stars, based on 1 article reviews
ap02242su n - by Bioz Stars, 2026-10
91/100 stars
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kit antibody  (Rockland Immunochemicals)
93
Rockland Immunochemicals kit antibody
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Kit Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/NFkB+Antibody+Sampler+Kit/pmc08831987-111-8-23
Average 93 stars, based on 1 article reviews
kit antibody - by Bioz Stars, 2026-10
93/100 stars
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rabbit anticollagen type ii  (Cedarlane)
85
Cedarlane rabbit anticollagen type ii
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Rabbit Anticollagen Type Ii, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Anti-Mouse+Glucagon+like+peptide+2%2C+Affinity+Purified%2C+(Polyclonal)%2C+(Rabbit+IgG)/pmc02761953-88-16-20
Average 85 stars, based on 1 article reviews
rabbit anticollagen type ii - by Bioz Stars, 2026-10
85/100 stars
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glucagon boster m00678 1 ihc  (Boster Bio)
90
Boster Bio glucagon boster m00678 1 ihc
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Glucagon Boster M00678 1 Ihc, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Anti-GLP1%2FGCG+Rabbit+Monoclonal+Antibody/pmc08495450__EMMM___13___e11668___s009-87-72-73
Average 90 stars, based on 1 article reviews
glucagon boster m00678 1 ihc - by Bioz Stars, 2026-10
90/100 stars
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rabbit anti glucagon antibody  (Boster Bio)
90
Boster Bio rabbit anti glucagon antibody
A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 <t>and</t> <t>MAFA</t> 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. <t>PYY.</t> M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.
Rabbit Anti Glucagon Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Anti-Glucagon+GCG+Rabbit+Monoclonal+Antibody/pmc06459929-20-0-6
Average 90 stars, based on 1 article reviews
rabbit anti glucagon antibody - by Bioz Stars, 2026-10
90/100 stars
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hepcidin  (Boster Bio)
91
Boster Bio hepcidin
(A) Prussian blue staining evaluated iron accumulation. (B) Iron accumulation significantly decreased in the TAA-SY group compared with the TAA group. (C) Western blot assays <t>of</t> <t>ferritin</t> and <t>hepcidin</t> protein expression. (D) Ferritin in the TAA-SY group was decreased compared with the TAA group. Hepcidin in the TAA-SY group was slightly increased compared with the TAA group. (E) T 2 * values and iron overload (%) were negatively correlated (* p <0.05, ** p <0.01, *** p <0.001). Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TAA, thioacetamide-injected; TAA-SY, TAA-injected and silymarin-treated.
Hepcidin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Anti-Hepcidin+HAMP+Rabbit+Monoclonal+Antibody/pmc09634766-55-15-17
Average 91 stars, based on 1 article reviews
hepcidin - by Bioz Stars, 2026-10
91/100 stars
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ck15  (Aviva Systems)
91
Aviva Systems ck15
Illustration of differential protein expression in samples with opposite relative amounts of UDH lesions. Magnified sections of representative 2‐D PAGE gels run with lysates from non‐malignant tissue samples containing (A) low or (B) higher relative levels of UDHs are shown. Gels were visualized by silver nitrate staining. Arrows indicate the positions of a differentially expressed protein (red arrow) and reference proteins (CK14, CK19, <t>CK15</t> and β‐actin; black arrows).
Ck15, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+peptide+antibody/Rabbit+anti+C-peptide+Antibody/pmc05527921-164-66-77
Average 91 stars, based on 1 article reviews
ck15 - by Bioz Stars, 2026-10
91/100 stars
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Image Search Results


A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 and MAFA 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. PYY. M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.

Journal: Gene therapy

Article Title: Stable insulin secreting ducts formed by reprogramming of cells in the liver using a three gene cocktail and a PPAR agonist

doi: 10.1038/gt.2013.50

Figure Lengend Snippet: A. EdU labeling (green) of SOX9-positive cells (lilac). B. Transformed ductal structures are positive for insulin, and EdU at 1 week (green). C–E. Transformed insulin-positive ductal structures express PDX1, NGN3 and MAFA 1 week after Ad-PNM delivery (insulin red; transcription factors green). F–H. The ductal structures still express PDX1 and MAFA, but not NGN3, 6 weeks after Ad-PNM delivery. D=duct. Scale bars 100μm, each horizontal set is the same magnification. The results are representative of at least three different samples. I–R. Characterization of the insulin positive ductal structures at 6 weeks. In each case insulin is red and the named product green. I. C-peptide. J. Somatostatin. K. Glucagon. L. PYY. M. E-caherin (ECAD). N. Cytokeratin 19 (CK19). O EpCAM. P. CD133. Q. OV6 (negative). R. Albumin (negative). S,T. Counts of insulin-positive cells in the liver parenchyma and in the ducts at one and six weeks after Ad-PNM delivery. At 1 week more insulin positive cells are found in the liver parenchyma than in ducts, but these cells are lost while those in the ducts persist.. Error bars are standard errors.

Article Snippet: The primary antibodies used were insulin 1:200 (Abcam), PDX1 1:1000 (Millipore), SOX9 1:5000 (Millipore), NGN3 1:100 (Santa Cruz), MAFA 1:100 (Santa Cruz), Somatostatin and Glucagon 1:250 (Dako), PYY 1:100 (Acris Antibodies), F4/80 1:100 (e-Biosciences), OV6 1: 100 (R& D Systems), CD-133 1:100 (Millipore), EpCAM 1:100 (Santa Cruz), ECAD 1:250 (eBiosciences), CK19 1:100 (Santa Cruz), C-Peptide 1:100 (Cell Signaling Technologies), Albumin 1:100 (Bethyl Laboratories).

Techniques: Labeling, Transformation Assay

(A) Prussian blue staining evaluated iron accumulation. (B) Iron accumulation significantly decreased in the TAA-SY group compared with the TAA group. (C) Western blot assays of ferritin and hepcidin protein expression. (D) Ferritin in the TAA-SY group was decreased compared with the TAA group. Hepcidin in the TAA-SY group was slightly increased compared with the TAA group. (E) T 2 * values and iron overload (%) were negatively correlated (* p <0.05, ** p <0.01, *** p <0.001). Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TAA, thioacetamide-injected; TAA-SY, TAA-injected and silymarin-treated.

Journal: Journal of Clinical and Translational Hepatology

Article Title: Preclinical Long-term Magnetic Resonance Imaging Study of Silymarin Liver-protective Effects

doi: 10.14218/JCTH.2021.00499

Figure Lengend Snippet: (A) Prussian blue staining evaluated iron accumulation. (B) Iron accumulation significantly decreased in the TAA-SY group compared with the TAA group. (C) Western blot assays of ferritin and hepcidin protein expression. (D) Ferritin in the TAA-SY group was decreased compared with the TAA group. Hepcidin in the TAA-SY group was slightly increased compared with the TAA group. (E) T 2 * values and iron overload (%) were negatively correlated (* p <0.05, ** p <0.01, *** p <0.001). Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TAA, thioacetamide-injected; TAA-SY, TAA-injected and silymarin-treated.

Article Snippet: Western blots reacted with primary antibodies against ferritin (3998S, Cell Signaling Technology, Beverly, MA, USA), hepcidin (M01347, Boster Biological Technology, Pleasanton, CA, USA) and GAPDH (ab8245, Abcam, USA), followed by HRP-conjugated secondary antibodies.

Techniques: Staining, Western Blot, Expressing, Control, Injection

Illustration of differential protein expression in samples with opposite relative amounts of UDH lesions. Magnified sections of representative 2‐D PAGE gels run with lysates from non‐malignant tissue samples containing (A) low or (B) higher relative levels of UDHs are shown. Gels were visualized by silver nitrate staining. Arrows indicate the positions of a differentially expressed protein (red arrow) and reference proteins (CK14, CK19, CK15 and β‐actin; black arrows).

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Illustration of differential protein expression in samples with opposite relative amounts of UDH lesions. Magnified sections of representative 2‐D PAGE gels run with lysates from non‐malignant tissue samples containing (A) low or (B) higher relative levels of UDHs are shown. Gels were visualized by silver nitrate staining. Arrows indicate the positions of a differentially expressed protein (red arrow) and reference proteins (CK14, CK19, CK15 and β‐actin; black arrows).

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Expressing, Staining

Immunophenotype of luminal cells in normal breast structures.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Immunophenotype of luminal cells in normal breast structures.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Marker

Nuclear receptor status in the various CK15/CK19 cell populations. Indirect four color immunofluorescence analysis of serial tissue sections from a normal breast specimen reacted with antibodies against cytokeratin 15 (CK15, Alexa Fluor® 488; green channel), cytokeratin 19 (CK19, Alexa Fluor® 594; red channel), and (A) ERα or (B) PgR (ER or PgR, Alexa Fluor® 633; blue channel), and counterstained with the nuclear stain DAPI (grey channel). (C–F) Illustrative images of acinar structures stained with CK15 (Alexa Fluor® 488; green channel), ERα (Alexa Fluor® 594; red channel), and cytokeratin 19 (Alexa Fluor® 633; blue channel) showing varying degrees of hyperplasia. (G) A low magnification view of a ductal tree illustrating the various cellular phenotypes observed and the negative correlation between ERα and CK15. Scale bar, 100 μm. In all cases only merged images are shown.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Nuclear receptor status in the various CK15/CK19 cell populations. Indirect four color immunofluorescence analysis of serial tissue sections from a normal breast specimen reacted with antibodies against cytokeratin 15 (CK15, Alexa Fluor® 488; green channel), cytokeratin 19 (CK19, Alexa Fluor® 594; red channel), and (A) ERα or (B) PgR (ER or PgR, Alexa Fluor® 633; blue channel), and counterstained with the nuclear stain DAPI (grey channel). (C–F) Illustrative images of acinar structures stained with CK15 (Alexa Fluor® 488; green channel), ERα (Alexa Fluor® 594; red channel), and cytokeratin 19 (Alexa Fluor® 633; blue channel) showing varying degrees of hyperplasia. (G) A low magnification view of a ductal tree illustrating the various cellular phenotypes observed and the negative correlation between ERα and CK15. Scale bar, 100 μm. In all cases only merged images are shown.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Immunofluorescence, Staining

Negative correlation of nuclear receptor status and CK15 expression. Indirect four color immunofluorescence analysis of a normal breast specimen reacted with antibodies against ERα (ER, Alexa Fluor® 488; green channel), PgR (PgR, Alexa Fluor® 594; red channel), and cytokeratin 15 (CK15, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). (C and D) Enlarged regions of the sections presented in A and B panels, respectively, illustrating the negative correlation between (C) ERα or (D) PgR, and CK15. Scale bar, 50 μm. In all cases only merged images are shown.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Negative correlation of nuclear receptor status and CK15 expression. Indirect four color immunofluorescence analysis of a normal breast specimen reacted with antibodies against ERα (ER, Alexa Fluor® 488; green channel), PgR (PgR, Alexa Fluor® 594; red channel), and cytokeratin 15 (CK15, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). (C and D) Enlarged regions of the sections presented in A and B panels, respectively, illustrating the negative correlation between (C) ERα or (D) PgR, and CK15. Scale bar, 50 μm. In all cases only merged images are shown.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Expressing, Immunofluorescence, Staining

IHC analysis of serial sections of paraffin‐embedded normal breast tissue stained with antibodies against (A) CK15, (B) CK19, (C) DRP3, (D) c‐KIT, (E) CK5 and (F) CK14. The red arrows in (E) indicate CK5 negative acini.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: IHC analysis of serial sections of paraffin‐embedded normal breast tissue stained with antibodies against (A) CK15, (B) CK19, (C) DRP3, (D) c‐KIT, (E) CK5 and (F) CK14. The red arrows in (E) indicate CK5 negative acini.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Staining

Indirect four color immunofluorescence analysis of lactating alveoli reacted with antibodies against cytokeratin 15 (CK15, Alexa Fluor® 488; green channel) P‐Stat5a (P‐STAT5a, Alexa Fluor® 594; red channel), casein (casein, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). Lactating breast tissue samples presented (A) undifferentiated CK15+/P‐Stat5a−/casein− cells present occasionally within lactating alveoli or in transitional structures. In a few cases (B) one could observe CK15+/P‐Stat5a+/casein− cells.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Indirect four color immunofluorescence analysis of lactating alveoli reacted with antibodies against cytokeratin 15 (CK15, Alexa Fluor® 488; green channel) P‐Stat5a (P‐STAT5a, Alexa Fluor® 594; red channel), casein (casein, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). Lactating breast tissue samples presented (A) undifferentiated CK15+/P‐Stat5a−/casein− cells present occasionally within lactating alveoli or in transitional structures. In a few cases (B) one could observe CK15+/P‐Stat5a+/casein− cells.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Immunofluorescence, Staining

Immunohistochemical analysis of large collecting ducts of the nipple in normal breast tissue. Serial tissue sections of normal breast specimens were stained with antibodies against (A) CK15, (B) CK19, (C) DRP3, (D) c‐KIT, (E) CK5, and (F) CK14 allowing the immunophenotyping of cells present at the collecting ducts.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Immunohistochemical analysis of large collecting ducts of the nipple in normal breast tissue. Serial tissue sections of normal breast specimens were stained with antibodies against (A) CK15, (B) CK19, (C) DRP3, (D) c‐KIT, (E) CK5, and (F) CK14 allowing the immunophenotyping of cells present at the collecting ducts.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Immunohistochemical staining, Staining

Immunophenotyping of luminal cells in UDHs. IHC analysis of serial tissue sections of normal breast tissue containing DRP3 positive UDHs. Serial tissue sections were stained with antibodies against DRP3, CK15 and CK19, allowing identification of three major DRP3+ cellular phenotypes: (panel A) DRP3+ (sub‐figure A)/CK15+ (sub‐figure B)/CK19+ (sub‐figure C), (panel B) DRP3+ (sub‐figure A)/CK15+ (sub‐figure B)/CK19− (sub‐figure C), and (panel C) DRP3+ (sub‐figure A)/CK15− (sub‐figure B)/CK19+ (sub‐figure C). Red arrows indicate cells with these phenotypes.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Immunophenotyping of luminal cells in UDHs. IHC analysis of serial tissue sections of normal breast tissue containing DRP3 positive UDHs. Serial tissue sections were stained with antibodies against DRP3, CK15 and CK19, allowing identification of three major DRP3+ cellular phenotypes: (panel A) DRP3+ (sub‐figure A)/CK15+ (sub‐figure B)/CK19+ (sub‐figure C), (panel B) DRP3+ (sub‐figure A)/CK15+ (sub‐figure B)/CK19− (sub‐figure C), and (panel C) DRP3+ (sub‐figure A)/CK15− (sub‐figure B)/CK19+ (sub‐figure C). Red arrows indicate cells with these phenotypes.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Staining

Immunophenotyping of columnar cell changes. (A and B) Indirect four color immunofluorescence analysis of tissue sections containing CCCs with antibodies against (A) cytokeratin 15 (CK15, Alexa Fluor® 488; green channel), ERα (ER, Alexa Fluor® 594; red channel), and cytokeratin 19 (CK19, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel) and (B) against c‐KIT (c‐KIT, Alexa Fluor® 488; green channel), vimentin (Vim, Alexa Fluor® 594; red channel), and cytokeratin 19 (CK19, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). (C–F) Immunohistochemical analysis of CCCs. Serial tissue sections of normal breast specimens were stained with antibodies against (C) CK15, (D) c‐KIT, (E) CK5, and (F) CK14 to immunophenotype cells present at CCCs.

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: Immunophenotyping of columnar cell changes. (A and B) Indirect four color immunofluorescence analysis of tissue sections containing CCCs with antibodies against (A) cytokeratin 15 (CK15, Alexa Fluor® 488; green channel), ERα (ER, Alexa Fluor® 594; red channel), and cytokeratin 19 (CK19, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel) and (B) against c‐KIT (c‐KIT, Alexa Fluor® 488; green channel), vimentin (Vim, Alexa Fluor® 594; red channel), and cytokeratin 19 (CK19, Alexa Fluor® 633; blue channel), counterstained with the nuclear stain DAPI (grey channel). (C–F) Immunohistochemical analysis of CCCs. Serial tissue sections of normal breast specimens were stained with antibodies against (C) CK15, (D) c‐KIT, (E) CK5, and (F) CK14 to immunophenotype cells present at CCCs.

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining

IHC analysis of serial sections of paraffin‐embedded normal breast tissue stained with antibodies against (A) ERα, (B) CK15, (C) c‐KIT, (D) CK19 and (E) PD‐ECGF, also known as endothelial cell growth factor (ECGF).

Journal: Molecular Oncology

Article Title: Tissue proteomics of the human mammary gland: Towards an abridged definition of the molecular phenotypes underlying epithelial normalcy

doi: 10.1016/j.molonc.2010.09.005

Figure Lengend Snippet: IHC analysis of serial sections of paraffin‐embedded normal breast tissue stained with antibodies against (A) ERα, (B) CK15, (C) c‐KIT, (D) CK19 and (E) PD‐ECGF, also known as endothelial cell growth factor (ECGF).

Article Snippet: Commercially available antibodies against the following antigens were used in this study (stated working dilutions are for IHC): CK5 (rabbit monoclonal clone EP 1601 Y; used at a dilution of 1:100) from EPITOMICS; CK8 (mice culture supernatant M20; used at a dilution of 1:200) from Monosan; CK14 (clone LL002; used at a dilution of 1:200), CK19 (mouse monoclonal; used at a dilution of 1:1000) from Labvision‐ThermoScientific; CK15 (rabbit polyclonal anti‐peptide; used at a dilution of 1:12000) from AVIVA Systems Biology; c‐Kit (rabbit polyclonal anti‐peptide A4502; used at a dilution of 1:500), ERα (mouse monoclonal clone 1D5; used at a dilution of 1:500), PgR (mouse monoclonal clone PgR 636; used at a dilution of 1:200), p63 (mouse monoclonal clone AA4; used at a dilution of 1:200), vimentin (mouse monoclonal V9; used at a dilution of 1:500) and CD44 (mouse monoclonal clone DF 1485; used at a dilution of 1: 200) from Dako (Glostrup, Denmark); TUC‐4 protein (rabbit polyclonal anti‐peptide AB5454; used at a dilution of 1:2000), from Millipore, phospho STAT5a –Y694 (rabbit polyclonal antibody; used at a dilution of 1:300) from ABGENT; beta‐casein (used at a dilution of 1200) from AbD Serotec; thymidine phosphorylase (mouse monoclonal clone P‐GF.44C; used at a dilution of 1:300) from IMGENEX, DRP3 (anti‐DYSPL3, HPA010948; used at a dilution of 1:500) from Atlas Antibodies AB, and CRMP1 (rabbit polyclonal anti‐peptide used at a dilution of 1:200) from Abcam.

Techniques: Staining